Detection of Aeromonas salmonicida in wild Atlantic salmon using a specific DNA probe test

نویسندگان

  • Jenny Mooney
  • Eithne Powell
  • Catherine Clabby
  • Richard Powell
چکیده

The presence of Aeromonas salrnonicida, the etiological agent of furunculosis, was examined using polymerase chain reaction (PCR) and DNA probe methodology in wild Atlantic salmon Salmo salar taken from 3 Irish river systems Blood samples from 61 individual fish were examined and the presence of pathogen was determined in 87% of the fish examined. However, the level of pathogen was extremely low, calculated at less than 100 A. salmonicida genome equivalents per fish. These data imply a widespread low level infection of A. salmonicida amongst wild salmon in Irish rivers.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

EPIDEMIOLOGICAL ASPECTS OF Aeromonas salmonicida IN THE MARINE ENVIRONMENT

The epidemiology of Aeromonas salmonicida subsp. salmonicida in the marine environment was investigated. Nutrient resuscitation and infectivity studies did not support a previous claim of dormancy in A. salmonicida and validated the use of colony-forming units (cfu) in survival studies. Survival of A. salmonicida in seawater was assessed and found to be of short duration «10 days). Survival of ...

متن کامل

Defined deletion mutants demonstrate that the major secreted toxins are not essential for the virulence of Aeromonas salmonicida.

The importance of the two major extracellular enzymes of Aeromonas salmonicida, glycerophospholipid: cholesterol acyltransferase (GCAT) and a serine protease (AspA), to the pathology and mortality of salmonid fish with furunculosis had been indicated in toxicity studies. In this study, the genes encoding GCAT (satA) and AspA (aspA) have been cloned and mutagenized by marker replacement of inter...

متن کامل

Monitoring of Aeromonas salmonicida in Chum salmon (Oncorhynchus keta) from Namdae River, Korea

In this study, 665 chum salmon (Oncorhynkus keta) were caught from Namdae river during 2006 and 2010, and monitored for typical Aeromonas salmonicida, a causative bacterium of furunculosis. 440 adults and 225 artificially hatched fry pools were examined by PCR using the typical A. salmonicida-specific vapA gene primers. The results demonstrated that 43.2% of the samples (287/665 samples) were P...

متن کامل

The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor.

Infections by the bacterium Aeromonas salmonicida subsp. achromogenes cause significant disease in a number of fish species. In this study, we showed that AsaP1, a toxic 19-kDa metallopeptidase produced by A. salmonicida subsp. achromogenes, belongs to the group of extracellular peptidases (Aeromonas type) (MEROPS ID M35.003) of the deuterolysin family of zinc-dependent aspzincin endopeptidases...

متن کامل

Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection

The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (350...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2006